Skygen Severe Fever with Thrombocytopenia Syndrome Virus Probe qRT-PCR Kit is designed for the quantitative detection of its specific RNA in a real-time RT-PCR test using hydrolysis probes. Purified RNA is reverse transcribed to cDNA and subsequently amplified using a pair of primers.In the amplification process, Taq DNA polymerase amplifies the cDNA sample via quantitative PCR using a primer-probe set that targets its specific cDNA region. At each cycle of the PCR, the 5’ nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples. This Probe qRT-PCR Kit has the following features: 1.One-tube RT-PCR，minimized carry-over contamination. 2.High sensitivity，with an analytical LOD at 100 copies/reaction. 3.High specificity，no cross-reaction with RNA from other pathogens. 4.Amplification positive control is provided in the kit. 5.Compatible with various RNA purification methods. 6.Suitable for both qualitative and quantitative detection. For quantitative use, the linear range is at least 5 orders of magnitude. 7.Enough for 50 reactions，20 μL per reaction. 12.Research use only.